How can I prepare a stock solution from a plant extract?
Tanta University. 1- First, a Beaker or a crucible should be brought, weighed (Assume that the weight is 100 g) 2- Transfer the extract to the Beaker. 3- The solvent used in the extraction process ...
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Antibacterial and antibiofilm activity of acetone leaf extracts …
The minimum inhibitory concentration of the plant extracts was determined. The lower the MIC is the better is the activity . Antimicrobial activity of plant extracts have been classified as good (MIC< 0.1 mg/mL), moderate (0.1 ≤ MIC ≤0.625 mg/mL) and weak (MIC > 0.625 mg/mL) . Most of the extracts had good activity against at least two of ...
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Analytical Methods Used in Determining Antioxidant …
The DPPH test is commonly applied to assess the antioxidant activity of plant extracts. To test the possible borate interference on the DPPH test, X. Chen et al. (2020) studied the effects of borates (0–16 mM sodium tetraborate) on the radical neutralisation capacity of Gallic acid (GA, at 10, 20 and 40 mg/L).
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Plant Extracts: Antimicrobial Properties, Mechanisms of Action …
It is noteworthy that plant extracts contain different types of bioactive compounds with antimicrobial activity for the utilization in food preservation (refer to Sects. 2 and 3). Different bioactives have different mechanisms of antimicrobial action due to their structure, their concentration, the feature of targeted microorganisms and ...
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Frontiers | Plant Extracts Rich in Polyphenols as Potent …
The concentration of the plant extracts varied in the range from 0.016 to 20 mg/mL. Subsequently, 10 μL of microbial suspension was added to each well to achieve a final concentration of 5 × 10 6 CFU/mL. Lastly, 10 μL of the resazurin solution (32.7 mM) was pipetted into all wells. Every plate contained a negative and a positive control.
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Plant Extract
Plant extracts are extremely complex, multicomponent mixtures and the degree of difficulty in accurate quantification of plant hormones is determined mainly by their low concentration in extracts (Hooykaas et al., 1999). As we know, endogenous plant hormones are present at trace amounts in plants, ...
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Phytochemical Screening and Antimicrobial Activity …
Minimum inhibitory concentration (MIC) values of the extracts and fractions were determined using broth micro dilution method. 30–33 Eppendorf tube was filled with 1gm of samples including the crude extracts and fractions of each plant. About 1 mL of dimethyl sulfoxide (DMSO) was added to each tube containing plant extracts.
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Potent Cytotoxicity of Four Cameroonian Plant Extracts on …
To investigate whether the apoptosis effect induced by the four studied plant extracts is dependent upon the caspases activation, the caspase 3/7 activity examined on HeLa cells treated with the concentration of IC 50 values of the four studied extracts for 24 h . DP expressed a significant effect on activation of caspase 3/7 up to 7.17 ± 0.72 ...
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Antibacterial activities of the extracts, fractions and isolated
Minimum inhibitory concentration (MIC) determination by microtitre broth dilution method: MIC was performed on all extracts, fractions and isolated compounds. The MIC of the plant extracts was determined by serial dilution, as described by Eloff according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Stock solutions of ...
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Antimicrobial activity, phytochemical screening of …
The search for plant extracts with highly antimicrobial activity has been increased nowadays. This study evaluated the antimicrobial activity of Pulicaria crispa …
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How do you determine the concentration of plant extract?
Most recent answer. the concentration of plant extract was added to metal salt precursor can determined using spectrophotometric method by followed the addtion of plant extract gradually, salt ...
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Minimum inhibitory concentrations of medicinal plants …
Results. The ethanolic extracts of 51 species inhibited Escherichia coli, and 114 ethanolic extracts inhibited Staphylococcus aureus. In contrast, only 30 aqueous extracts showed activity against E. coli and 38 extracts against S. aureus. The MIC concentrations were mostly very high and ranged from 0.008 to 256mg/ml, with only 36 …
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Optimization of Glycerol–Water Extraction of Selected …
An increase in the concentration of glycerol to 50%, 65% and 80%, respectively, however, brought about a decrease in the concentration of polyphenols to 372.4 mg GAE/L, 340.7 mg GAE/L and 276.7 mg GAE/L. Peppermint leaf extraction with glycerol–water systems at temperature of 50 °C and 20 °C was also characterised by a …
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A Sensitive and Quick Microplate Method to Determine the …
A Sensitive and Quick Microplate Method to Determine the Minimal Inhibitory Concentration of Plant Extracts for Bacteria @article{Eloff1998ASA, title={A Sensitive and Quick Microplate Method to Determine the Minimal Inhibitory Concentration of Plant Extracts for Bacteria}, author={Jacobus Nicolaas Eloff}, …
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Antimicrobial activity of some plant extracts against …
The concentration effect of the effective plant extracts were reported in Table 3 and illustrated in Fig. 2. The inhibitory effect of P. granatum extract started at 2.5 mg/ml with inhibition zones of 9.6 and 8.3 mm against S. aureus and P. aeruginosa while extract of S. aromaticum suppressed bacterial growth of these strains at concentration …
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In-vitro analysis of free radical scavenging activities and suppression
The lethal concentration at 50% (LC 50), which is the lowest concentration of the extracts that inhibits 50% of the cells, was calculated using the trendline coefficient with the goodness of fit ...
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Antimicrobial Properties and Mechanism of Action of Some Plant Extracts …
One ml of plant extracts with a final concentration of 20% (w/v), were added to 1 ml of bacteria or cell-free filtrate. Fluorescence intensities were determined after 10 min by fluorescence spectrophotometer ( 4500, Japan), using excitation wavelengths of 490 nm and emission wavelengths of 520 nm. While the excitation slits widths were 5 ...
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Plant Extracts: Antimicrobial Properties, Mechanisms …
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(PDF) Plant extracts: antimicrobial and antifungal activity …researchgate(PDF) Antibacterial activity of some plant extractsWeb23 rowsPlant extracts are extremely complex, multicomponent mixtures and the degree of difficulty in accurate quantification of plant hormones is determined mainly by their …
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Antioxidant Activity as an Indicator of the Efficiency of Plant …
Several researchers have documented that the use of different plant species [13,14], different parts of the plant and different plant extract concentrations [16,17] can affect the morphology and particle size of the obtained ZnO [6,18]. To the best of our knowledge, little has been said about the purity and yield of the final product, which are ...
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Antibacterial Activity of Four Plant Extracts Extracted from
To conduct the time–kill assay using the in vitro model, each plant extract was added to 1mL cecum solution to obtain 1/2, 1, 2, 3, and 4 × MIC of each plant extract. Concentrations were based on the broth microdilution results which identified the minimum concentrations of plant extracts that inhibit bacteria in broth after 24 h.
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In vitro antimicrobial efficacy of Cassia alata (Linn.) leaves, …
Agar-well diffusion assay. Table 1 shows the average diameter of inhibition zones produced by C. alata crude extracts from both maceration and Soxhlet extraction tested in several concentrations with different plant's parts and extraction solvents. The results showed that ethyl acetate extracts from both maceration and Soxhlet extraction …
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ANTIOXIDANT AND ANTIFUNGAL ACTIVITY OF SELECTED MEDICINAL PLANT
Results: All plant extracts were active against the selected plant phytopathogenic fungi. Moreover, Bucida buceras had the best antifungal activity against four of the fungi, with minimum inhibitory concentration (MIC) values as low as 0.02 mg/ml and 0.08 mg/ml against P. expansum, P. janthinellum, T. harzianum and F. …
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Challenges to Antimicrobial Susceptibility Testing of Plant …
Screening crude plant extracts for desired bioactivity is one of the most important operations in the study of medicinal plants, and extraction is the first crucial step. The quality of plant extracts depends on plant material (part of the plant used fresh or dried), which may vary with climate, time of harvest, extraction method, solvent used ...
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Phytochemical Screening, Antioxidant, and Antimicrobial …
The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of the extracts were determined by the broth microdilution method . Briefly, each plant extract (8192 μ g/mL) was serially diluted two-fold with MHB in a 96-well microplate at a total volume of 100 µ L.
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Effects of Varying Concentrations of the Crude Aqueous and …
Cold (room temperature; 26 to 29 °C) and hot water extracts of each plant part were prepared by steeping 40 g each, of the powdered plant material in 1L of the water, respectively for 24 hrs, followed by filtration. 4000 mg/l stock concentrations of the extracts were prepared by dilution of the filtrates with the appropriate volumes of ...
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Plant-Derived Antioxidants: Significance in Skin Health and …
The concentration of α-tocopherol was significantly lower in the epidermis of photoaged and aged skin, ... Plant extracts not only have the ability to scavenge free radicals, but also support the defence and regenerative mechanisms of the skin [8,9,17,18,22,24]. 4. The Antioxidant Activity of Plants
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Assessments of Antibacterial Effects of Aqueous-Ethanolic Extracts …
Based on the mean value zone of inhibition, the plant extracts' antibacterial activity ability was depending on the concentrations of the plant extracts used. At the concentration of 62.5mg/ml of plant extract, S. aureus had no inhibition zone, whereas other bacteria show little mean zone of inhibition diameter in the same concentration (62 ...
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Antibacterial activity and phytochemical components of leaf …
When used at a concentration of 75 mg/ml, the crude extract had similar antibacterial effects as gentamicin (0.1 mg/ml) and the ethanol fraction. ... research on the safety of plant extracts in ...
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Phenolic-Rich Plant Extracts With Antimicrobial Activity: An
Addition of plant extracts at concentrations exceeding 1% (w/w) are generally required to effectively inhibit the growth of unwanted microorganisms in foods. It might even be more promising to add phenolic-rich plant extracts in foods containing lower amounts of food constituents interacting with proteins and fat, ...
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Phytochemicals: Extraction, Isolation, and Identification …
It has been reported that ethanolic extracts of Ivorian plants extracted higher concentrations/amount of phenolics compared to acetone, water, and methanol . Multiple solvents have been commonly used to extract phytochemicals, and scientists usually employed a dried powder of plants to extract bioactive compounds and eliminate …
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