A simple method for high molecular-weight genomic DNA extraction
Optimized genomic DNA extraction protocol. (A) Agarose gel electrophoresis of gDNA samples obtained with the DNeasy Plant Mini Kit (Qiagen) or with our protocol. For both methods, three replicates are presented. (B) Size distribution of extracted genomic DNA measured with a Fragment Analyzer. The multi-peak profile is …
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A phenol/chloroform-free method to extract nucleic acids …
Background Woody tropical plants contain high levels of complex organic compounds that inhibit the chemical procedures needed to extract RNA or DNA, thus compromising downstream applications such as RNA sequencing and analysis of gene expression. To overcome this issue, researchers must use extraction protocols using …
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EXTRACTION OF GENOMIC DNA FROM YEASTS FOR PCR-BASED APPLICATIONS
In summary, we have developed a quick and reliable method for gDNA extraction from yeasts that is suitable for PCR amplification of DNA fragments up to 3500 bp. The protocol can be carried out in a single test tube within 15 minutes and cells from both liquid and solid media can be used. The method is suitable for routine genotyping of yeasts ...
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Improved high-molecular-weight DNA extraction, nanopore
Short-read metagenomic sequencing and de novo genome assembly of the human gut microbiome can yield draft bacterial genomes without isolation and culture. However, bacterial genomes assembled from ...
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Optimized DNA extraction and purification method for
Quality and quantity of genomic DNA from five extraction protocols. Lung tissue specimens from seven autopsied individuals were processed for DNA extraction following the five methods described ...
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Current Nucleic Acid Extraction Methods and Their …
The method can be used to extract DNA from bacteria, although a large-scale culture is needed ; this method can be used for purification of various forms of DNA ... Wang Z., Stenger D. A. Magnetic bead-based solid phase for selective extraction of genomic DNA. Analytical Biochemistry. 2006; 355 (2):285–297. doi: 10.1016/j.ab.2006.05.005 ...
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DNA Extraction | Genomic DNA Isolation
Promega genomic DNA extraction products are designed for isolation of high-quality DNA from many sample types, including blood, cells, tissues, plants, microbes and more. DNA extraction kits are available for all sample throughput needs–from simple manual systems through benchtop automation to customizable chemistries for DNA isolation on ...
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Methods for extracting genomic DNA from whole blood …
Diego Chacon-Cortes, Lyn R Griffiths Genomics Research Centre, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD, Australia Abstract: Deoxyribonucleic acid (DNA) extraction has considerably evolved since it was initially performed back in 1869. It is the first step required for many of the available …
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Sequencing 101: DNA extraction — tips, kits, and protocols
In this introduction to DNA extraction, we will share tips, tricks, and protocols to help make your DNA isolation easier. For optimal results to power biological discovery, sample prep is a critical step in any sequencing project. And with long-read sequencing technologies, including HiFi sequencing, you not only want DNA free from …
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Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform
With bacterial genomic DNA extraction kits, such as the WIZARD Genomic DNA Purification Kit (Promega), the multiple active reagents are added as a sequence of proprietary solutions with vague names such as "Nuclei Lysis Solution" or "Protein Precipitation Solution." Though somewhat informative, these titles offer no information ...
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DNA Extraction Protocols | Thermo Fisher Scientific
DNA Extraction from Blood. DNA Extraction from Buccal Swabs. DNA Extraction from Serum. DNA Extraction from Tissue. Dynabeads DNA DIRECT Blood. Dynabeads DNA DIRECT Universal. Dynabeads Streptavidin Trial Kit. Enhanced Automated Immunomagnetic Separation (eAIMS) for Escherichia coli O157. Extraction of DNA …
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DNeasy Blood & Tissue Kits
Product Details. DNeasy Blood & Tissue Kits provide fast, easy silica-based DNA extraction without phenol or chloroform in convenient spin-column and 96-well-plate formats. Most samples can be directly lysed with proteinase K, eliminating the need for mechanical disruption and reducing hands-on time. Optimized protocols for specific …
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Genomic DNA Extraction by Sample Type
Isolate and purify high-quality genomic DNA from a wide variety of sample types, including tissue, cells, blood, serum, plants, and forensic samples. Whether you prefer organic reagents, filter columns, or magnetic beads, …
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Genomic DNA Extraction & Purification | NEB
The Monarch Genomic DNA Purification Kit ( NEB #T3010) is a comprehensive kit for gDNA extraction and purification from a wide variety of sample types including blood, cells, tissues, and tough-to-lyse samples including bacteria and yeast. This kit includes lysis buffers for various input materials, Proteinase K for homogenization of some ...
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Genomic DNA (gDNA) Extraction and Purification | NEB
The Monarch Genomic DNA Purification Kit is a universal kit for DNA extraction and purification from a wide variety of cell types, including blood, cells, tissues and tough-to-lyse samples (including bacteria and yeast). The kit includes lysis buffers for various input materials, Proteinase K for homogenization of some sample types, and RNase A ...
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Comparison of commercial DNA extraction kits for whole …
For example, DNA extraction is crucial to the success of microbiome sequencing and is known to introduce bias in 16S studies [3 – 6]. The wide variations of cell membrane structures and composition can pose significant challenges to efficiency and bias-free extraction of genomic DNA.
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A single protocol for extraction of gDNA from bacteria and …
Guanidine thiocyanate breakage of microorganisms has been the standard initial step in genomic DNA (gDNA) extraction of microbial DNA for two decades, despite the requirement for pretreatments to extract DNA from microorganisms other than Gram-negative bacteria. We report a quick and low-cost gDNA extraction protocol called EtNa …
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A Simple Method of Genomic DNA Extraction from Human …
The successful sample collection and the extraction of genomic DNA from buccal swabs, urine, and hair are noninvasive and reliable alternatives to the prickly invasive blood sampling, both for subjects and sample collectors. 17 – 20 We have demonstrated here a simple and novel method of the sample collection and DNA extraction, which is cost ...
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A simple plant high-molecular-weight DNA extraction …
Background High-molecular-weight and pure DNA is crucial for high-quality results from 3rd generation DNA Analyzers and optical mapping technologies. Conventional nuclei isolation methods for preparing high-molecular-weight genomic DNA from plant tissues include the preparation of protoplasts or embedding nuclei in an agarose matrix …
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DNA Purification | DNA Extraction Methods
Another specialized genomic DNA isolation system is the Wizard® Magnetic DNA Purification System for Food (.# FF3750, FF3751). This convenient protocol is designed for the manual purification of DNA from a …
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Genomic DNA Extraction and Genomic DNA …
Genomic DNA extraction is the process of releasing chromosomal DNA from the cellular matrix in which it is contained. Genomic DNA extraction requires a robust …
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A new, simple, highly scalable, and efficient protocol for genomic DNA …
The major goal of this study is the development of a rapid and simple extraction method capable of yielding large amounts of high‐quality genomic DNA that is suitable for use with common laboratory techniques such as PCR and short‐read sequencing (e.g., Illumina or BGI‐Seq).
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Comparison of three genomic DNA extraction methods to obtain high DNA
The genomic DNA was extracted following CTAB-based method used to extract DNA from seeds of soybean, wheat, barley, oats, maize, and rice [9, 10]. This method took about 3–4 h for 10 samples. This method took about 3–4 h for 10 samples.
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Genomic DNA Extraction & Purification | NEB
NEB offers two approaches for the extraction and purification of gDNA: the Monarch Genomic DNA Purification Kit, which is a silica spin column based process and the …
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Optimized DNA extraction and purification method …
Moreover, high concentration of human genomic DNA (gDNA) compared to microbial DNA 5 can inhibit the PCR. Thus, it is crucial to develop an efficient method of …
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Quick Protocol for Extraction and Purification of Genomic DNA
Store RNase A and Proteinase K at -20°C. Add ethanol (≥ 95%) to the gDNA Wash Buffer concentrate as indicated on the bottle label. Set a thermal mixer (e.g. ThermoMixer ®) or, if not available, a heating block to 56°C for sample lysis. Set a heating block to 60°C. Preheat the appropriate volume of elution buffer to 60°C (35-100 μl per ...
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Advances in point-of-care nucleic acid extraction technologies for
For example, for genomic DNA isolation, white blood cells need to be separated from the rest of the blood components (J. Choi et al., 2015). In contrast, for the detection of pathogenic nucleic acids or cell-free DNAs, either serum or pathogen-infected blood cells are separated before extraction ( Zhang et al., 2019 ).
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DNA extraction — Science Learning Hub
This activity outlines how to extract the DNA from a tomato. What does DNA extraction involve? Step 1. Breaking cells open to release the DNA. The cells in a sample are separated from each other, often by a physical means such as grinding or vortexing, and put into a solution containing salt. The positively charged sodium ions in the salt help ...
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Genomic DNA (gDNA) Extraction and Purification | NEB
The Monarch Genomic DNA Purification Kit is a universal kit for DNA extraction and purification from a wide variety of cell types, including blood, cells, tissues and tough-to …
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Comparison of six methods for Loa loa genomic …
Objectives Good-quality and sufficient DNA is essential for diagnostics and vaccine development. We aimed to compare six DNA extraction techniques applied to Loa loa microfilariae in order to …
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